Dr. Ainsely Lewis, PhD Postdoctoral fellow, Department of Biology, Trent University

“Welcome to the wild wild west in metabolomics – a peek into Metabolomics workflow.”

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Date: May 18, 5 to 6pm ET

ABSTRACT:

All organisms go through metabolism. All cells go through biochemical reactions within metabolism and the byproducts thereof are known as metabolites. Metabolomics is the large-scale study of small molecules also known as metabolites within the cells, biofluids, or tissues of organisms. A compendium of metabolites within an organism is known as the ‘metabolome’. To investigate the metabolome, mass spectrometry is used to measure the masses of all the molecules that would be present in samples of a given organism. Being a relatively new field, compared to the sister fields of genomics, transcriptomics, and proteomics, metabolomics is used as a tool for finding environmental cues of organisms, especially for changes within the metabolome at a given time or under specific conditions. Metabolomics is a rapidly evolving field, especially with an increasing number of tools available for both those experienced in programming or not. Despite this trend, compared to the other ‘omics’ fields, that have established protocols and workflows, the workflow for metabolomics is still not standardized. This adds to the challenges that stands in the way of covering the metabolome. For this talk, the focus will be on mass spectrometry-based metabolomics and workflow from the perspective of a plant laboratory group.

The CBSN/RCSN gratefully acknowledges the University of Toronto Scarborough and its support as the Network Host Institution, and the Academic Program Committee of the Network for the BE-STEMM Seminar Series.